Original Run Volume 1: in chapter 1, two female characters are sexually harassed in a saloon. In chapter 4, a child claims they needed to get away from an uncle who was "into sodomy". The story is revealed to be made-up. Volume 2: in chapter 17, three women are brought into a cafe by a group of men stated to be slave traffickers and referred to as "perverts". The women are made to sit on the floor and are in collars and chains. One of them is struck when she is caught looking at another man. The man in question frees the women after forcing the slavers to kill themselves. In chapter 19, the main character pops the buttons on a femail antagonist's shirt and says that if he wanted to he could have groped her five times. This is in response to her earlier removal of his coat buttons and claim that she could have killed him three times. In chapter 20, the main antagonist (the protagonist's twin brother) attacks the protagonist in an attempt to force him to use his angel arm. The antagonist is naked throughout the scene and holds the protagonist from behind with a hand over his mouth. The protagonist remembers a "sick" feeling from the last time he faced his brother. During the scene the protagonist screams and cries while the antagonist asks "how do you like it?" The scene is not sexual in nature but resembles a sexual assault. Volume 2 pilot: the main character attempts to look up a woman's skirt as she is suspended from the ceiling. Trigun Maximum: Volume 1: in chapter 1, a 12-year-old girl says an adult male antagonist touched her butt and refers to him as a "lolicon". The protagonist refuses to send her out to the gang and instead obeys the gang leader's instruction to strip and act like a dog. The protagonist is shot while naked in the street. The girl is abducted by the gang. The main protagonist and second main male character rescue her. In chapter 6, a character describes the rape and murder of his daughter at the hands of the man he is threatening to kill. Volume 4: in chapter 23, a male character has a woman captive and states that she will become his thirteenth bride. She tries to fight him off and it is implied he has killed the previous twelve women. The main female characters rescue her. Volume 5: in chapter 29, characters in a flashback assume that a catatonic woman is a male character's "toy". He is actually her caregiver. In chapter 30, the main character regains his memory of the events alluded to in volume 2 of the original run. This occurs as a traumatic flashback. In this flashback he remembers his brother forcing him to use his power, which the brother describes in a way reminiscent of orgasm. He touches the protagonist's face and lays him on his back. When the protagonist resists, he pushes him down and climbs on top of him. He succeeds in drawing out the protagonist's power, which destroys a city. The protagonist remains lost in this traumatic flashback for a few chapters. The confrontation is not sexual in nature but it is framed like a sexual assault. Volume 7: in chapter 45, the protagonist and antagonist face off again and again the protagonist ends up on his back, with his brother standing over him. This time he is penetrated with one of his brother's knife appendages. The scene is not sexual, but the framing is reminiscent of a rape. Volume 8 (spinoff): the second main male character rescues a female character from a forced marriage. The male character she was going to be forced to marry becomes angry at her perceived loss of value when he thinks that she is no longer a virgin. Volume 9: in chapter 51, a male bandit implies he will rape a captive girl and then says to the boy trying to rescue her that he will "pass her" to him when he "is done." The bandit is killed by a main male character. In chapter 53, during a flashback, a teenage boy is cornered by several adult men and it is implied that they may intend to sexually assault him. He is shown being beaten, but the scene cuts away. Nothing sexual is confirmed. His alter eventually kills the men. Volume 10: in chapter 57, a male antagonist, who has previously been shown to have raised and trained two of the main male characters and been referred to as a threat to children at an orphanage, attacks one of the male characters. The protagonist is on his knees and the antagonist uses his gun to tip the protagonist's chin up. He then pins the protagonist to the ground with the weapon. He stabs the barrel into the character's chest, leaving the tip of the gun bloodied when he draws it out and thrusts it back in. His cloak is shown between the prone protagonist's trembling legs. The scene is not sexual, but the imagery could imply a prior abusive relationship. The characters were part of a religious cult in which the older man had complete authority over the young boys he was training. Some of the antagonist's dialogue could also be read as sexually charged, especially given the common symbolic use of guns as stand-ins for male sexual organs. He is depicted emotionally manipulating the other male character in a flashback, leaving this character emotionally dependent on him. Volume 12: in chapter 79, a flashback reveals that one of the male antagonists was a child sex slave before he was rescued by the main antagonist. There is a graphic depiction of his owner raping him. The rapist is cut into pieces by the main antagonist.